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2 edition of Molecular genetics of cellulase production by Xanthomonas campestris. found in the catalog.

Molecular genetics of cellulase production by Xanthomonas campestris.

Clare Linda Gough

Molecular genetics of cellulase production by Xanthomonas campestris.

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Published by University of EastAnglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D), University of East Anglia, School of Biological Sciences, 1989.

ID Numbers
Open LibraryOL13925550M

  Xa21, the first R-gene cloned from rice, confers resistance to a broad range of strains of Xanthomonas oryzae pv. oryzae (Ishiyama) Swings et al. [= X. campestris pv. oryzae (Ishiyama) Dye] (Xoo) (Song et al., ). Xa21 was isolated by map-based cloning and found to be a member of a complex locus located on chromosome 11 (Song et al., ).   Molecular analysis of a gene that affects extracellular polysaccharide production and virulence in Pseudomonas solanacearum.- Interactions between Arabidopsis thaliana and phytopathogenic Pseudomonas pathovars: A model for the genetics of disease resistance.- Interaction between Arabidopsis thaliana and Xanthomonas campestris   Gboyega E. Adebami, Bukola C. Adebayo-Tayo, Development of cellulolytic strain by genetic engineering approach for enhanced cellulase production, Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass, /B, (), (). Colombian isolates were found to be related toXcampestris pv. cassavae (7), X campestris pv. manihotis (5a, 15), or X campestris pv. poinsettiicola (33). Deviant X campestris pv. cassavae has been isolated from cassava leaf spots in Niger (13), recently clustered inXcampestris pv. cassavae (34, 38). Molecular approaches based onDNApolymorphisms.

Section II: Molecular Genetics of Phytopathogenic Bacteria and Fungi.- "Cutinase and pectinase in host-pathogen and plant-bacterial interaction".- "Siderophore biosynthesis, uptake and effect on potato growth of rhizosphere strains".- "A gene cluster in Xanthomonas campestris PV Campestris required for pathogenicity controls the excretion of.


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Molecular genetics of cellulase production by Xanthomonas campestris. by Clare Linda Gough Download PDF EPUB FB2

Molecular genetics of cellulase production by Xanthomonas campestris. (Thesis) Gough CL. Publisher: University of East Anglia [] Metadata Source: The British Library Type: Thesis.

Abstract. No abstract supplied. Menu. Formats. Abstract. EThOS. About. About Europe PMC; Funders; Joining Europe PMC Cited by: 1. Ghasem D.

Najafpour, in Biochemical Engineering and Biotechnology, Example 1. Xanthan gum is produced using Xanthomonas campestris in a batch culture.

Laboratory experiments have shown that for each gram of glucose utilised by the bacteria, g oxygen and g ammonia are consumed while g gum, g cells, g gaseous CO 2 and g H 2 O are formed.

Molecular genetics of cellulase production by Xanthomonas campestris. Author: Gough, Clare Linda. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text.

The nucleotide sequence of a kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB.

The genes xanA and xanB encode proteins of amino acids (molecular weight of 48,) and amino acids (molecular weight of 50,), respectively. The kinetics of growth and xanthan production by Xanthomonas campestris ATCC in batch culture were studied in a laboratory fermenter without pH control.

Fermentations were carried out over a range of Molecular genetics of cellulase production by Xanthomonas campestris. book speeds (– rpm) and the pyruvate content, as well as the molecular weight of the product were by: Xanthomonas campestris is bacterial species that causes a variety of plant diseases, including "black rot" in cruciferous vegetables and bacterial wilt of turfgrass.

It is also used in the commercial production of xanthan gum, a high-molecular-weight polysaccharide which has many important uses, especially in the food industry. The major aim of this research was to investigate interactions between the bacterial pathogen Xanthomonas campestris pv.

raphani (Xcr) and Arabidopsis thaliana which are largely unexplored as a model pathosystem, and to identify genetic loci conferring resistance to this pathogen. Xcr is genetically close to X.

campestris pv. campestris (Xcc) and both pathogens infect common Brassicaceae. The identification of Xanthomonas-like colonies was performed by molecular and in vivo tests. According to Vicente description (), the strains with vascular behavior were identified as X.

campestris and were detected in the 6% of the analysed seed samples. Xanthomonas campestris produced xanthan yield of g/l using cidified molasses and a acidified aerated molasses. This fermentation study has an advantage over some other manufacturing processes with its use of agro industrial wastes as the raw material, allowing the increased xanthan production.

Keywords. Xanthomonas campestris. caused by Xanthomonas oryzae pv. oryzac (Swings et al., ) has been an important constraint to rice production in Asia (Lozano, ). In Pakistan, this disease was reported first during (Mew and Majid, ). The bacterium can infect rice from seedling stage. Xanthomonas wilt of enset and banana was first described in Ethiopia in and is now a serious disease that is spreading in East and Central Africa.

Earlier studies identified Xanthomonas campestris pv. musacearum as the causal agent. This paper describes the characterisation of strains of the bacterium that were isolated in four countries. Abstract. The title of this chapter is not wholly accurate.

Our primary purpose is not to describe Xanthomonas genetics as a topic per se but rather to illustrate the use of genetic approaches to analyse aspects of Xanthomonas biology. Most of the studies reported so far have been concerned either with phytopathogenicity or with the production of polysaccharide.

A Gene Cluster in Xanthomonas campestris pv campestris Required for Pathogenicity Controls the Excretion of Enzymes. Pages Possibly Disturbed in the Production of a Compound Required for the Induction of Nitrogenase Activity in Bacteroids.

Pages Postma, Jenne G. (et al.) Book Title Molecular Genetics of Plant-Microbe. Xanthomonas campestris cell–cell signalling molecule DSF One such evolutionarily well-conserved bacterial process is the production of cell–cell signalling molecules which regulate production of multiple virulence functions by a process known as quorum sensing.

cellulase (mg ml –1), or buffer (10mM potassium phosphate buffer. Introduction. Xanthomonas campestris tris (Xcc), the causal agent of black rot of crucifers, is an important bacterial pathogen capable of infecting all the members of the cabbage family (Schaad and Alvarez, ).

Xcc produces a range of extracellular enzymes (including protease, cellulase, polygalacturonate lyase, lipase and amylase) and extracellular polysaccharide (EPS), which. Gough C L () Molecular genetics of cellulase production by Xanthomonas campestris. PhD Thesis, University of East Anglia, UK.

PhD Thesis, University of East Anglia, UK. Google Scholar. The gram-negative plant-pathogenic species Xanthomonas campestris has two common traits, yellow pigmentation and exopolysaccharide (xanthan gum) production which render the colonies yellowish and mucoid.

This species consists of more than pathovars, which can be differentiated on the basis of the host plants they infect ().Most of the pathovars exhibit high degrees of host specificity in. A description is provided for Xanthomonas campestris pv. begoniae.

Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On begonias. Reiger-type are most susceptible, but the disease is also reported on rhizomatous, fibrous, tuberous and winter flowering hybrid varieties (Harri et al., ; 54, ).

Xanthomonas campestris pv. vesicatoria (Xcv), the causal agent of bacterial spot on tomatoes and pepper, provides an excellent model system to study the molecular basis of host specificity.

Xcv (Race 2) specifically induces a hypersensitive reaction on culturing of pepper containing the monogene dominant resistance gene Bs(1). Thenucleotide sequence ofa kb EcoRI-PstI DNAfragment ofXanthomonas campestris pv. campestyis revealed twoopenreadingframes, whichweredesignatedxanAandxanB.

ThegenesxanAandxanBencode proteins of aminoacids (molecular weightof48,) and aminoacids (molecular. Xanthomonas campestris pv campestris (Xcc) is a plant pathogenic bacterium that controls the production of pathogenicity factors in part by a cluster of genes designated rpf (regulation of.

Xanthan gum is an extracellular heteropolysaccharide produced by the bacterium Xanthomonas campestris. Xanthan has wide commercial application as a viscosifier of aqueous solutions. Previously, through genetic engineering, a set of mutants defective in.

Many plant-pathogenic bacteria utilize type II secretion (T2S) systems to secrete degradative enzymes into the extracellular milieu. T2S substrates presumably mediate the degradation of plant cell wall components during the host-pathogen interaction and thus promote bacterial virulence.

Previously, the Xps-T2S system from Xanthomonas campestris pv. vesicatoria was shown to contribute to. Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is a disease of crucifer crops. The objective of this study was to characterize races of Xcc, their distribution and genetic diversity in India.

Two hundred and seventeen isolates of bacteria were obtained from 12 different black rot‐infected crucifer crops from 19 states of India; these were identified as Xcc based on.

Xanthomonas colonies are yellow, slightly mucoid, mounded and circular in shape. Xanthomonas utilizes Tween and in days a white crystalline halo usually forms around the yellow colony. Some strains of Xanthomonas form only a weak halo or only clear the medium under the colony (not visible).

The colony size and color can differ within a sample. Molecular Plant-Microbe Interactions ® (MPMI) publishes fundamental and advanced applied research on the genetics, genomics, molecular biology, biochemistry, and biophysics of pathological, symbiotic, Complete Genome Sequence of Strain WHRI Race 1 of Xanthomonas campestris pv.

campestris, the Causal Agent of Black Rot of Cruciferous. Cellulase Enzyme Production Activity. Cellulase production was determined by using the method of Wood et al.

The isolates were spot inoculated into the medium containing NaNO 3 1 g, K 2 HPO 4 1 g, KCl 1 g, MgSO 4 g, FeSO 4 g, yeast extract 5 g, Agar 15 g, carboxy methyl cellulose (CMC) 2 g and incubated for 2 days at 28˚C. After cell. Background In Citrus cultures, three species of Xanthomonas are known to cause distinct diseases.

citri subsp. citri patothype A, X. fuscans subsp. aurantifolii pathotypes B and C, and X. alfalfae subsp. citrumelonis, are the causative agents of cancrosis A, B, C, and citrus bacterial spots, respectively.

Although these species exhibit different levels of virulence and aggressiveness, only. This book, divided into 13 chapters, explores recent discoveries in the area of molecular plant-microbe interactions. It focuses mainly on the mechanisms controlling plant disease resistance and the cross talk among the signalling pathways involved, and the strategies used by fungi and viruses to suppress these defences.

Two chapters deal with the role of symbionts (such as the symbiotic 5/5(2). Xanthan production by the local strain Xanthomonas campestris ICL2 was studied in shake flasks and bioreactor as a batch culture. Inoculation of modified Garcia-Ochoa's medium with 2% cell suspension and incubation at 30°C were the best treatments for xanthan production after 6 days in batch shake flasks experiments.

The absence of organic acid had a bad effect on cell growth and xanthan. Introduction. Based on DNA‐DNA homology values, the Xanthomonas genus comprises at least 20 different species (Vauterin et al., ).

Xanthomonas campestris belongs to DNA group 15 and is one of the most dominant species within the genus. It consists of several pathovars identified by classical taxonomic methods (Dye et al., ), which, in most cases, can be differentiated on the.

Results. Xanthomons campestris pathovar campestris (Xcc) is the causal agent of black rot disease of cruciferous this study, a cDNA library was constructed from the low-molecular weight RNA isolated from the Xcc strain grown to exponential phase in the minimal medium sRNA candidates were obtained by sequencing screen of 2, clones from the library and four of them.

Inthe Daniels Laboratory in the UK discovered that plant pathogen Xanthomonas campestris itris (Xcc) could produce a diffusible signaling factor (DSF) and regulate production of a. Xanthomonas campestris tris (Xcc), the causal agent of black rot in crucifers, produces a membrane‐bound yellow pigment called xanthomonadin to protect against photobiological and peroxidative damage, and uses a quorum‐sensing mechanism mediated by the diffusible signal factor (DSF) family signals to regulate virulence factors production.

Journal of bacteriology, ISSN03/, VolumeIssue 5, pp. - Molecular Plant-Microbe Interactions ® (MPMI) publishes fundamental and advanced applied research on the genetics, genomics, molecular biology, biochemistry, and biophysics of pathological, symbiotic, Xanthomonas campestris Promotes Diffusible Signal Factor Biosynthesis and Pathogenicity by Utilizing Glucose and Sucrose from Host Plants.

Natural Genetic Variation of Xanthomonas campestris pv. campestris Pathogenicity on Arabidopsis Revealed by Association and Reverse Genetics Endrick Guy,a,b Anne Genissel, a,b* Ahmed Hajri,c* Matthieu Chabannes, * Perrine David,c* Sébastien Carrere,a,b Martine Lautier,a,b,d Brice Roux,a,b Tristan Boureau,e Matthieu Arlat,a,b,d Stéphane Poussier,f* Laurent D.

Noëla,b. Molecular Microbiology 4. Qihong Sun, Jun Hu, Guixiu Huang, Chao Ge, Rongxiang Fang and Chaozu He (). The type II secretion pathway structural gene xpsE is required for xylanase- and cellulase-secretion and virulence in Xanthomonas oryzae pv.

oryzae. Plant Pathology 5. Buttner D, Lorenz C, Weber E, Bonas U (): Targeting of two effector protein classes to the type III secretion system by a HpaC - and HpaB-dependent protein complex from Xanthomonas campestris pv. vesicatoria. Molecular Microbiology, 59, – doi: /jx. This book provides a state-of-the art overview on microbial cyclic di-nucleotide signaling with a the facultative human pathogen Pseudomonas aeruginosa, global plant pathogens as exemplified by Xanthomonas campestris and Vincent T.

Lee is professor of Cell Biology and Molecular Genetics at the University of Maryland at College Park, USA. Fig. 2. Absorption spectra of the methanol extracts of X. campestris PTCC (1) and mutant XCE 2 (2). Pigmentation. Colonies of the genus Xanthomonas are yellow in color due to the production of membrane-bound brominated aryl polyene pigments called Xanthomonadins [37].

Since such pigmentation is unique to this genus, it is commonly used as a marker for chemotaxonomy [37].Infiltration of cellulase (EC [][1]) from Trichoderma longibrachiatum into melon (Cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms.

An oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (SA) signaling pathways leading to marked induction of peroxidase and chitinase.Biology, biochemistry and ultrastructure of Xanthomonas-plant interactions; MAMPs and defence elicitation; Xanthan and defence suppression; Infection of .